In many cases, the separation of highly polar compounds requires highly aqueous mobile phase conditions to achieve suffi cient retention on the stationary phase. Conventional reversed phase selectivities do not give reproducible results under these conditions due to mainly collapse of the C18 chains. Therefore, YMC did developed Hydrosphere C18 in order of to overcome the loss in retention. Now, this outstanding chromatographic behaviour has been transferred to YMC-UltraHT Hydrosphere C18.
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