Ascentis Express HILIC

– 15 columns
Vendor: Sigma Aldrich

Ascentis® Express HILIC HPLC Column
2.7 μm particle size, L × I.D. 10 cm × 2.1 mm    

Ascentis® Express HILIC HPLC Columns
Suitable for L3 per USP
HILIC chromatography is a variation of normal-phase chromatography with the distinction that one of the major components of the mobile phase is water. The high volatility of the mobile phase makes HILIC LC-MS friendly, and allows for increased MS sensitivity. Many classes of polar compounds can be retained on the Ascentis™ Express HILIC column; including, polar neutrals, polar acids, and polar and non-polar basic amines. The HILIC column provides orthogonal selectivity to the Ascentis Express C18, with the elution typically the opposite of that for C18. The non-polar compounds elute early and the highly polar compounds have greater retention and selectivity.

Ascentis Express HPLC columns, through the use of Fused-Core™ particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users.

Brand specifications

Every parameter recorded for every column in this Brand is shown here. A single value applies across the Brand; multiple values show what is recorded across its columns.

Mode Reversed-Phase Operating Temperature 100
Modification Silica pH stability from 2.0
Pore Size (Å) 90 pH stability to 8.0
Endcapped No

Choose column specifications

Available: 30 – 150 mm
Available: 2.1 – 4.6 mm
Particle Size Recorded value 2.7 μm
Pore Size Recorded value 90 Å

Columns in this brand

Use the selectors above to narrow the list by specification, or search here by P/N or any displayed value.

15 columns shown
53933-U 30 2.1 2.7 90
53934-U 50 2.1 2.7 90
53938-U 75 2.1 2.7 90
53939-U 100 2.1 2.7 90
53946-U 150 2.1 2.7 90
53964-U 30 3.0 2.7 90
53967-U 50 3.0 2.7 90
53969-U 75 3.0 2.7 90
53970-U 100 3.0 2.7 90
53972-U 150 3.0 2.7 90
53974-U 30 4.6 2.7 90
53975-U 50 4.6 2.7 90
53977-U 75 4.6 2.7 90
53979-U 100 4.6 2.7 90
53981-U 150 4.6 2.7 90
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