Discovery HS F5
– 36 columns
Discovery® HS F5 HPLC Column
3 μm particle size, L × I.D. 10 cm × 2.1 mm
Discovery® HS F5
Suitable for L43 per USP
Features and Benefits
- Unique selectivity
- Similar retention to C18 (sometimes requires stronger mobile phase)
- Excellent peak shape
- Stable, low-bleed LC-MS separations
- Scalable separations from 3 to 10μm particle sizes
Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
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Brand specifications
Every parameter recorded for every column in this Brand is shown here. A single value applies across the Brand; multiple values show what is recorded across its columns.
| Mode | Reversed-Phase | Endcapped | Yes |
|---|---|---|---|
| Modification | Silica/PFP | Operating Temperature | 70 |
| Pore Size (Å) | 120 | pH stability from | 2.0 |
| Surface Area | 300 | pH stability to | 8.0 |
| Carbon Load, % | 12.0 |
Choose column specifications
Columns in this brand
Use the selectors above to narrow the list by specification, or search here by P/N or any displayed value.
| 567500-U | 33 | 2.1 | 3.0 | 120 |
| 567501-U | 33 | 2.1 | 3.0 | 120 |
| 567502-U | 100 | 2.1 | 3.0 | 120 |
| 567503-U | 150 | 2.1 | 3.0 | 120 |
| 567504-U | 50 | 4.6 | 3.0 | 120 |
| 567505-U | 33 | 3.0 | 3.0 | 120 |
| 567506-U | 100 | 4.6 | 3.0 | 120 |
| 567507-U | 150 | 4.6 | 3.0 | 120 |
| 567508-U | 50 | 2.1 | 5.0 | 120 |
| 567509-U | 33 | 4.6 | 3.0 | 120 |
| 567510-U | 100 | 2.1 | 5.0 | 120 |
| 567511-U | 150 | 2.1 | 5.0 | 120 |
| 567512-U | 250 | 2.1 | 5.0 | 120 |
| 567513-U | 50 | 4.6 | 5.0 | 120 |
| 567515-U | 100 | 4.6 | 5.0 | 120 |
| 567516-U | 150 | 4.6 | 5.0 | 120 |
| 567517-U | 250 | 4.6 | 5.0 | 120 |
| 567518-U | 50 | 10.0 | 5.0 | 120 |
| 567519-U | 150 | 10.0 | 5.0 | 120 |
| 567520-U | 250 | 10.0 | 5.0 | 120 |
| 567521-U | 50 | 21.2 | 5.0 | 120 |
| 567522-U | 150 | 21.2 | 5.0 | 120 |
| 567523-U | 250 | 21.2 | 5.0 | 120 |
| 567528-U | 150 | 21.2 | 10.0 | 120 |
| 567529-U | 250 | 21.2 | 10.0 | 120 |
| 567530-U | 50 | 4.0 | 3.0 | 120 |
| 567531-U | 100 | 4.0 | 3.0 | 120 |
| 567532-U | 150 | 4.0 | 3.0 | 120 |
| 567533-U | 50 | 4.0 | 5.0 | 120 |
| 567534-U | 100 | 4.0 | 5.0 | 120 |
| 567535-U | 150 | 4.0 | 5.0 | 120 |
| 567536-U | 250 | 4.0 | 5.0 | 120 |
| 567537-U | 100 | 10.0 | 5.0 | 120 |
| 567540-U | 100 | 21.2 | 10.0 | 120 |
| 567541-U | 150 | 3.0 | 5.0 | 120 |
| 567542-U | 150 | 3.0 | 3.0 | 120 |