NUCLEOSIL PEI
– 1 column
Vendor: MACHEREY-NAGEL
NUCLEOSIL® standard silica phases for HPLC
Features
NUCLEOSIL® is a family of totally porous spherical silicas with a very pure and uniform SiO2 structure
- Wide acceptance as routine packings for very different fields of chromatography
- One of the first spherical silicas used in HPLC
- Developed in the early seventies, it became a world-renowned HPLC packing
- Still found in many analytical and preparative applications
- An absolutely reliable choice in HPLC
-
The largest variety of modified HPLC silicas available on the market
NUCLEOSIL® 4000-7 PEI
Silica-based HPLC phase for anion exchange chromatography of proteins and peptides
Features
- Base material NUCLEOSIL® silica, particle size 7 µm, pore size 4000 Å polymeric, covalently bonded polyethyleneimine network, weakly basic anion exchanger
- Ion exchange capacity 0.15 mmol/g; protein binding capacity 61 mg BSA/g; pH stability 2–8.5; max. working pressure 250 bar
- Separation principle: reversible adsorption of negatively charged substances to positively charged groups on the exchanger material and their subsequent displacement by either increasing ionic strength or pH changes in the mobile phase
- Eluent in column methanol
- High selectivity for numerous proteins: e.g., β-lactoglobulins A and B, two proteins differing in just two amino acids, can be separated in only 10 minutes; biological activity of purified proteins is preserved
- Good binding and desorption kinetics for nucleotides as well
Select an image to enlarge and zoom.
Brand specifications
Every parameter recorded for every column in this Brand is shown here. A single value applies across the Brand; multiple values show what is recorded across its columns.
| Mode | Anion-Exchange | Max Pressure | 3625 |
|---|---|---|---|
| Modification | Silica/WAX | Operating Temperature | 60 |
| Pore Size (Å) | 4000 | pH stability from | 2.0 |
| Surface Area | 10 | pH stability to | 8.5 |
| Endcapped | N/A |
Choose column specifications
Length
Recorded value
125 mm
Internal Diameter
Recorded value
4 mm
Particle Size
Recorded value
7 μm
Pore Size
Recorded value
4000 Å
Columns in this brand
Use the selectors above to narrow the list by specification, or search here by P/N or any displayed value.
1 columns shown
| 720402.4 | 125 | 4.0 | 7.0 | 4000 |
No columns match these filters. Clear one or more filters to see other options.