PolyETHYL A
– 70 columnsPolyETHYL A - for Hydrophobic Interaction Chromatography
HIC materials separate proteins on the basis of hydrophobic character, as does RPC. However, HIC uses totally aqueous buffers, retaining tertiary structure and biological activity. Selectivity is generally superior to that of RPC for proteins and polypeptides large enough to have significant secondary or tertiary structure. Typically, a sample is eluted with a decreasing gradient of a salt such as a sulfate or phosphate. Proteins elute in order of increasing surface hydrophobicity. Surfactants (e.g., CHAPS; octylglucoside) can be added to the mobile phase if necessary. HIC has greater sensitivity than other modes to the location of a modification or residue difference, especially (but not necessarily) if it involves a nonpolar group.
Use these HIC materials for:
- Multidimensional protein purification (suggested sequence: Ion-exchange - [add salt to collected fractions] - HIC).
- Purification of polypeptides (e.g., venoms and glycopeptides).
- Characterization of antibodies.
- QC analysis of proteins differing in the polarity of a single residue or modified positional variants.
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Brand specifications
Every parameter recorded for every column in this Brand is shown here. A single value applies across the Brand; multiple values show what is recorded across its columns.
| Mode | HIC | Pore Size (Å) | 1000 / 1500 / 300 |
|---|---|---|---|
| Modification | Silica/ Polyethyl | Operating Temperature | Ambient |
Choose column specifications
Columns in this brand
Use the selectors above to narrow the list by specification, or search here by P/N or any displayed value.
| 050.05ET0503 | 50 | 0.05 | 5.0 | 300 |
| 050.05ET0510 | 50 | 0.05 | 5.0 | 1000 |
| 050.10ET0503 | 50 | 0.1 | 5.0 | 300 |
| 050.10ET0510 | 50 | 0.1 | 5.0 | 1000 |
| 050.17ET0503 | 50 | 0.175 | 5.0 | 300 |
| 050.17ET0510 | 50 | 0.175 | 5.0 | 1000 |
| 050.22ET0503 | 50 | 0.22 | 5.0 | 300 |
| 050.22ET0510 | 50 | 0.22 | 5.0 | 1000 |
| 050.32ET0503 | 50 | 0.32 | 5.0 | 300 |
| 050.32ET0510 | 50 | 0.32 | 5.0 | 1000 |
| 051ET0503 | 50 | 1.0 | 5.0 | 300 |
| 051ET0510 | 50 | 1.0 | 5.0 | 1000 |
| 054.0ET0503 | 50 | 4.0 | 5.0 | 300 |
| 054.0ET0510 | 50 | 4.0 | 5.0 | 1000 |
| 054ET0503 | 50 | 4.6 | 5.0 | 300 |
| 054ET0510 | 50 | 4.6 | 5.0 | 1000 |
| 100.05ET0503 | 100 | 0.05 | 5.0 | 300 |
| 100.05ET0510 | 100 | 0.05 | 5.0 | 1000 |
| 100.10ET0503 | 100 | 0.1 | 5.0 | 300 |
| 100.10ET0510 | 100 | 0.1 | 5.0 | 1000 |
| 100.17ET0503 | 100 | 0.175 | 5.0 | 300 |
| 100.17ET0510 | 100 | 0.175 | 5.0 | 1000 |
| 100.22ET0503 | 100 | 0.22 | 5.0 | 300 |
| 100.22ET0510 | 100 | 0.22 | 5.0 | 1000 |
| 100.32ET0503 | 100 | 0.32 | 5.0 | 300 |
| 100.32ET0510 | 100 | 0.32 | 5.0 | 1000 |
| 102ET0503 | 100 | 2.1 | 5.0 | 300 |
| 102ET0510 | 100 | 2.1 | 5.0 | 1000 |
| 104.0ET0503 | 100 | 4.0 | 5.0 | 300 |
| 104.0ET0510 | 100 | 4.0 | 5.0 | 1000 |
| 104ET0503 | 100 | 4.6 | 5.0 | 300 |
| 104ET0510 | 100 | 4.6 | 5.0 | 1000 |
| 109ET0503 | 100 | 9.4 | 5.0 | 300 |
| 109ET0510 | 100 | 9.4 | 5.0 | 1000 |
| 150.05ET0503 | 150 | 0.05 | 5.0 | 300 |
| 150.05ET0510 | 150 | 0.05 | 5.0 | 1000 |
| 150.10ET0503 | 150 | 0.1 | 5.0 | 300 |
| 150.10ET0510 | 150 | 0.1 | 5.0 | 1000 |
| 150.17ET0503 | 150 | 0.175 | 5.0 | 300 |
| 150.17ET0510 | 150 | 0.175 | 5.0 | 1000 |
| 150.22ET0503 | 150 | 0.22 | 5.0 | 300 |
| 150.22ET0510 | 150 | 0.22 | 5.0 | 1000 |
| 150.32ET0503 | 150 | 0.32 | 5.0 | 300 |
| 150.32ET0510 | 150 | 0.32 | 5.0 | 1000 |
| 151ET0503 | 150 | 1.0 | 5.0 | 300 |
| 151ET0510 | 150 | 1.0 | 5.0 | 1000 |
| 202ET0503 | 200 | 2.1 | 5.0 | 300 |
| 202ET0510 | 200 | 2.1 | 5.0 | 1000 |
| 204ET0503 | 200 | 4.6 | 5.0 | 300 |
| 204ET0510 | 200 | 4.6 | 5.0 | 1000 |
| 204ET1203 | 200 | 4.6 | 12.0 | 300 |
| 204ET1215 | 200 | 4.6 | 12.0 | 1500 |
| 209ET0503 | 200 | 9.4 | 5.0 | 300 |
| 209ET0510 | 200 | 9.4 | 5.0 | 1000 |
| 209ET1203 | 200 | 9.4 | 12.0 | 300 |
| 209ET1215 | 200 | 9.4 | 12.0 | 1500 |
| 2521ET0503 | 250 | 21.0 | 5.0 | 300 |
| 2521ET0510 | 250 | 21.0 | 5.0 | 1000 |
| 2521ET1203 | 250 | 21.0 | 12.0 | 300 |
| 2521ET1215 | 250 | 21.0 | 12.0 | 1500 |
| 2550ET1203 | 250 | 50.8 | 12.0 | 300 |
| 2550ET1215 | 250 | 50.8 | 12.0 | 1500 |
| 259ET0503 | 250 | 9.4 | 5.0 | 300 |
| 259ET0510 | 250 | 9.4 | 5.0 | 1000 |
| 259ET1203 | 250 | 9.4 | 12.0 | 300 |
| 259ET1215 | 250 | 9.4 | 12.0 | 1500 |
| 3.52ET0503 | 35 | 2.1 | 5.0 | 300 |
| 3.52ET0510 | 35 | 2.1 | 5.0 | 1000 |
| 3.54ET0503 | 35 | 4.6 | 5.0 | 300 |
| 3.54ET0510 | 35 | 4.6 | 5.0 | 1000 |