PolyHYDROETHYL A
– 215 columns
Vendor: Poly LC Inc.
PolyHYDROXYETHYL A - for Hydrophilic Interaction and Size Exclusion HPLC
This neutral, polar material was developed specifically for Hydrophilic Interaction Chromatography (HILIC). HILIC is a variant of normal phase chromatography that is performed with a polar stationary phase and a partly aqueous mobile phase. This permits normal-phase separations of peptides, nucleic acids, carbohydrates, some proteins, and polar solutes in general. In comparison with other materials used for HILIC, PolyHYDROXYETHYL A affords sharper peaks and better selectivity and recovery. Its great polarity means less organic solvent is needed to get retention.
Use PolyHYDROXYETHYL A for:
- Analysis of polar small molecules for metabolomics or analysis via HILIC-MS/MS of specific small molecules (amino acids; methotrexate) in plasma or crude extracts.
- Peptide separations and mapping involving differences in polar groups (Ser-; glycopeptides; etc.).
- Multidimensional purification of synthetic and natural peptides or fractionation of really complex digests.
- HPLC of solutes that aren't soluble in aqueous media (membrane proteins;phospholipids).
- Eliminating detergents, lipids, and salts from a sample.
- Oligonucleotides and their analogs.
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Brand specifications
Every parameter recorded for every column in this Brand is shown here. A single value applies across the Brand; multiple values show what is recorded across its columns.
| Mode | HILIC/Size Exclusion | Pore Size (Å) | 100 / 1000 / 1500 / 200 / 300 / 500 / 60 |
|---|---|---|---|
| Modification | Silica/ Polyhydroxyethyl | Operating Temperature | Ambient |
Choose column specifications
Available: 35 – 250 mm
Available: 0.05 – 50.8 mm
Available: 3 – 12 μm
Available: 60 – 1500 Å
Columns in this brand
Use the selectors above to narrow the list by specification, or search here by P/N or any displayed value.
215 columns shown
| 050.05HY05006 | 50 | 0.05 | 5.0 | 60 |
| 050.05HY0501 | 50 | 0.05 | 5.0 | 100 |
| 050.05HY0502 | 50 | 0.05 | 5.0 | 200 |
| 050.05HY0503 | 50 | 0.05 | 5.0 | 300 |
| 050.05HY0505 | 50 | 0.05 | 5.0 | 500 |
| 050.05HY0510 | 50 | 0.05 | 5.0 | 1000 |
| 050.10HY05006 | 50 | 0.1 | 5.0 | 60 |
| 050.10HY0501 | 50 | 0.1 | 5.0 | 100 |
| 050.10HY0502 | 50 | 0.1 | 5.0 | 200 |
| 050.10HY0503 | 50 | 0.1 | 5.0 | 300 |
| 050.10HY0505 | 50 | 0.1 | 5.0 | 500 |
| 050.10HY0510 | 50 | 0.1 | 5.0 | 1000 |
| 050.17HY05006 | 50 | 0.175 | 5.0 | 60 |
| 050.17HY0501 | 50 | 0.175 | 5.0 | 100 |
| 050.17HY0502 | 50 | 0.175 | 5.0 | 200 |
| 050.17HY0503 | 50 | 0.175 | 5.0 | 300 |
| 050.17HY0505 | 50 | 0.175 | 5.0 | 500 |
| 050.17HY0510 | 50 | 0.175 | 5.0 | 1000 |
| 050.22HY05006 | 50 | 0.22 | 5.0 | 60 |
| 050.22HY0501 | 50 | 0.22 | 5.0 | 100 |
| 050.22HY0502 | 50 | 0.22 | 5.0 | 200 |
| 050.22HY0503 | 50 | 0.22 | 5.0 | 300 |
| 050.22HY0505 | 50 | 0.22 | 5.0 | 500 |
| 050.22HY0510 | 50 | 0.22 | 5.0 | 1000 |
| 050.32HY05006 | 50 | 0.32 | 5.0 | 60 |
| 050.32HY0501 | 50 | 0.32 | 5.0 | 100 |
| 050.32HY0502 | 50 | 0.32 | 5.0 | 200 |
| 050.32HY0503 | 50 | 0.32 | 5.0 | 300 |
| 050.32HY0505 | 50 | 0.32 | 5.0 | 500 |
| 050.32HY0510 | 50 | 0.32 | 5.0 | 1000 |
| 051HY03006 | 50 | 1.0 | 3.0 | 60 |
| 051HY0301 | 50 | 1.0 | 3.0 | 100 |
| 051HY0305 | 50 | 1.0 | 3.0 | 200 |
| 051HY0315 | 50 | 1.0 | 3.0 | 300 |
| 051HY05006 | 50 | 1.0 | 5.0 | 500 |
| 051HY0501 | 50 | 1.0 | 5.0 | 1000 |
| 051HY0502 | 50 | 1.0 | 5.0 | 200 |
| 051HY0503 | 50 | 1.0 | 5.0 | 300 |
| 051HY0505 | 50 | 1.0 | 5.0 | 500 |
| 051HY0510 | 50 | 1.0 | 5.0 | 1000 |
| 054.0HY05006 | 50 | 4.0 | 5.0 | 60 |
| 054.0HY0501 | 50 | 4.0 | 5.0 | 100 |
| 054.0HY0502 | 50 | 4.0 | 5.0 | 200 |
| 054.0HY0503 | 50 | 4.0 | 5.0 | 300 |
| 054.0HY0505 | 50 | 4.0 | 5.0 | 500 |
| 054.0HY0510 | 50 | 4.0 | 5.0 | 1000 |
| 054HY05006 | 50 | 4.6 | 5.0 | 60 |
| 054HY0501 | 50 | 4.6 | 5.0 | 100 |
| 054HY0502 | 50 | 4.6 | 5.0 | 200 |
| 054HY0503 | 50 | 4.6 | 5.0 | 300 |
| 054HY0505 | 50 | 4.6 | 5.0 | 500 |
| 054HY0510 | 50 | 4.6 | 5.0 | 1000 |
| 100.05HY05006 | 100 | 0.05 | 5.0 | 60 |
| 100.05HY0501 | 100 | 0.05 | 5.0 | 100 |
| 100.05HY0502 | 100 | 0.05 | 5.0 | 200 |
| 100.05HY0503 | 100 | 0.05 | 5.0 | 300 |
| 100.05HY0505 | 100 | 0.05 | 5.0 | 500 |
| 100.05HY0510 | 100 | 0.05 | 5.0 | 1000 |
| 100.10HY05006 | 100 | 0.1 | 5.0 | 60 |
| 100.10HY0501 | 100 | 0.1 | 5.0 | 100 |
| 100.10HY0502 | 100 | 0.1 | 5.0 | 200 |
| 100.10HY0503 | 100 | 0.1 | 5.0 | 300 |
| 100.10HY0505 | 100 | 0.1 | 5.0 | 500 |
| 100.10HY0510 | 100 | 0.1 | 5.0 | 1000 |
| 100.17HY05006 | 100 | 0.175 | 5.0 | 60 |
| 100.17HY0501 | 100 | 0.175 | 5.0 | 100 |
| 100.17HY0502 | 100 | 0.175 | 5.0 | 200 |
| 100.17HY0503 | 100 | 0.175 | 5.0 | 300 |
| 100.17HY0505 | 100 | 0.175 | 5.0 | 500 |
| 100.17HY0510 | 100 | 0.175 | 5.0 | 1000 |
| 100.22HY05006 | 100 | 0.22 | 5.0 | 60 |
| 100.22HY0501 | 100 | 0.22 | 5.0 | 100 |
| 100.22HY0502 | 100 | 0.22 | 5.0 | 200 |
| 100.22HY0503 | 100 | 0.22 | 5.0 | 300 |
| 100.22HY0505 | 100 | 0.22 | 5.0 | 500 |
| 100.22HY0510 | 100 | 0.22 | 5.0 | 1000 |
| 100.32HY05006 | 100 | 0.32 | 5.0 | 60 |
| 100.32HY0501 | 100 | 0.32 | 5.0 | 100 |
| 100.32HY0502 | 100 | 0.32 | 5.0 | 200 |
| 100.32HY0503 | 100 | 0.32 | 5.0 | 300 |
| 100.32HY0505 | 100 | 0.32 | 5.0 | 500 |
| 100.32HY0510 | 100 | 0.32 | 5.0 | 1000 |
| 102HY03006 | 100 | 2.1 | 3.0 | 60 |
| 102HY0301 | 100 | 2.1 | 3.0 | 100 |
| 102HY0305 | 100 | 2.1 | 3.0 | 500 |
| 102HY0315 | 100 | 2.1 | 3.0 | 1500 |
| 102HY05006 | 100 | 2.1 | 5.0 | 60 |
| 102HY0501 | 100 | 2.1 | 5.0 | 100 |
| 102HY0502 | 100 | 2.1 | 5.0 | 200 |
| 102HY0503 | 100 | 2.1 | 5.0 | 300 |
| 102HY0505 | 100 | 2.1 | 5.0 | 500 |
| 102HY0510 | 100 | 2.1 | 5.0 | 1000 |
| 104.0HY05006 | 100 | 4.0 | 5.0 | 60 |
| 104.0HY0501 | 100 | 4.0 | 5.0 | 100 |
| 104.0HY0502 | 100 | 4.0 | 5.0 | 200 |
| 104.0HY0503 | 100 | 4.0 | 5.0 | 300 |
| 104.0HY0505 | 100 | 4.0 | 5.0 | 500 |
| 104.0HY0510 | 100 | 4.0 | 5.0 | 1000 |
| 104HY03006 | 100 | 4.6 | 3.0 | 60 |
| 104HY0301 | 100 | 4.6 | 3.0 | 100 |
| 104HY0305 | 100 | 4.6 | 3.0 | 500 |
| 104HY0315 | 100 | 4.6 | 3.0 | 1500 |
| 104HY05006 | 100 | 4.6 | 5.0 | 60 |
| 104HY0501 | 100 | 4.6 | 5.0 | 100 |
| 104HY0502 | 100 | 4.6 | 5.0 | 200 |
| 104HY0503 | 100 | 4.6 | 5.0 | 300 |
| 104HY0505 | 100 | 4.6 | 5.0 | 500 |
| 104HY0510 | 100 | 4.6 | 5.0 | 1000 |
| 109HY03006 | 100 | 9.4 | 3.0 | 60 |
| 109HY0301 | 100 | 9.4 | 3.0 | 100 |
| 109HY0305 | 100 | 9.4 | 3.0 | 500 |
| 109HY0315 | 100 | 9.4 | 3.0 | 1500 |
| 109HY05006 | 100 | 9.4 | 5.0 | 60 |
| 109HY0501 | 100 | 9.4 | 5.0 | 100 |
| 109HY0502 | 100 | 9.4 | 5.0 | 200 |
| 109HY0503 | 100 | 9.4 | 5.0 | 300 |
| 109HY0505 | 100 | 9.4 | 5.0 | 500 |
| 109HY0510 | 100 | 9.4 | 5.0 | 1000 |
| 150.05HY05006 | 150 | 0.05 | 5.0 | 60 |
| 150.05HY0501 | 150 | 0.05 | 5.0 | 100 |
| 150.05HY0502 | 150 | 0.05 | 5.0 | 200 |
| 150.05HY0503 | 150 | 0.05 | 5.0 | 300 |
| 150.05HY0505 | 150 | 0.05 | 5.0 | 500 |
| 150.05HY0510 | 150 | 0.05 | 5.0 | 1000 |
| 150.10HY05006 | 150 | 0.1 | 5.0 | 60 |
| 150.10HY0501 | 150 | 0.1 | 5.0 | 100 |
| 150.10HY0502 | 150 | 0.1 | 5.0 | 200 |
| 150.10HY0503 | 150 | 0.1 | 5.0 | 300 |
| 150.10HY0505 | 150 | 0.1 | 5.0 | 500 |
| 150.10HY0510 | 150 | 0.1 | 5.0 | 1000 |
| 150.17HY05006 | 150 | 0.175 | 5.0 | 60 |
| 150.17HY0501 | 150 | 0.175 | 5.0 | 100 |
| 150.17HY0502 | 150 | 0.175 | 5.0 | 200 |
| 150.17HY0503 | 150 | 0.175 | 5.0 | 300 |
| 150.17HY0505 | 150 | 0.175 | 5.0 | 500 |
| 150.17HY0510 | 150 | 0.175 | 5.0 | 1000 |
| 150.22HY05006 | 150 | 0.22 | 5.0 | 60 |
| 150.22HY0501 | 150 | 0.22 | 5.0 | 100 |
| 150.22HY0502 | 150 | 0.22 | 5.0 | 200 |
| 150.22HY0503 | 150 | 0.22 | 5.0 | 300 |
| 150.22HY0505 | 150 | 0.22 | 5.0 | 500 |
| 150.22HY0510 | 150 | 0.22 | 5.0 | 1000 |
| 150.32HY05006 | 150 | 0.32 | 5.0 | 60 |
| 150.32HY0501 | 150 | 0.32 | 5.0 | 100 |
| 150.32HY0502 | 150 | 0.32 | 5.0 | 200 |
| 150.32HY0503 | 150 | 0.32 | 5.0 | 300 |
| 150.32HY0505 | 150 | 0.32 | 5.0 | 500 |
| 150.32HY0510 | 150 | 0.32 | 5.0 | 1000 |
| 151HY05006 | 150 | 1.0 | 5.0 | 60 |
| 151HY0501 | 150 | 1.0 | 5.0 | 100 |
| 151HY0502 | 150 | 1.0 | 5.0 | 200 |
| 151HY0503 | 150 | 1.0 | 5.0 | 300 |
| 151HY0505 | 150 | 1.0 | 5.0 | 500 |
| 151HY0510 | 150 | 1.0 | 5.0 | 1000 |
| 202HY05006 | 200 | 2.1 | 5.0 | 60 |
| 202HY0501 | 200 | 2.1 | 5.0 | 100 |
| 202HY0502 | 200 | 2.1 | 5.0 | 200 |
| 202HY0503 | 200 | 2.1 | 5.0 | 300 |
| 202HY0505 | 200 | 2.1 | 5.0 | 500 |
| 202HY0510 | 200 | 2.1 | 5.0 | 1000 |
| 204HY05006 | 200 | 4.6 | 5.0 | 60 |
| 204HY0501 | 200 | 4.6 | 5.0 | 100 |
| 204HY0502 | 200 | 4.6 | 5.0 | 200 |
| 204HY0503 | 200 | 4.6 | 5.0 | 300 |
| 204HY0505 | 200 | 4.6 | 5.0 | 500 |
| 204HY0510 | 200 | 4.6 | 5.0 | 1000 |
| 204HY1201 | 200 | 4.6 | 12.0 | 100 |
| 204HY1203 | 200 | 4.6 | 12.0 | 300 |
| 204HY1215 | 200 | 4.6 | 12.0 | 1500 |
| 209HY05006 | 200 | 9.4 | 5.0 | 60 |
| 209HY0501 | 200 | 9.4 | 5.0 | 100 |
| 209HY0502 | 200 | 9.4 | 5.0 | 200 |
| 209HY0503 | 200 | 9.4 | 5.0 | 300 |
| 209HY0505 | 200 | 9.4 | 5.0 | 500 |
| 209HY0510 | 200 | 9.4 | 5.0 | 1000 |
| 209HY1201 | 200 | 9.4 | 12.0 | 100 |
| 209HY1203 | 200 | 9.4 | 12.0 | 300 |
| 209HY1215 | 200 | 9.4 | 12.0 | 1500 |
| 2521HY05006 | 250 | 21.0 | 5.0 | 60 |
| 2521HY0501 | 250 | 21.0 | 5.0 | 100 |
| 2521HY0502 | 250 | 21.0 | 5.0 | 200 |
| 2521HY0503 | 250 | 21.0 | 5.0 | 300 |
| 2521HY0505 | 250 | 21.0 | 5.0 | 500 |
| 2521HY0510 | 250 | 21.0 | 5.0 | 1000 |
| 2521HY1201 | 250 | 21.0 | 12.0 | 100 |
| 2521HY1203 | 250 | 21.0 | 12.0 | 300 |
| 2521HY1215 | 250 | 21.0 | 12.0 | 1500 |
| 2550HY1201 | 250 | 50.8 | 12.0 | 100 |
| 2550HY1203 | 250 | 50.8 | 12.0 | 300 |
| 2550HY1215 | 250 | 50.8 | 12.0 | 1500 |
| 259HY05006 | 250 | 9.4 | 5.0 | 60 |
| 259HY0501 | 250 | 9.4 | 5.0 | 100 |
| 259HY0502 | 250 | 9.4 | 5.0 | 200 |
| 259HY0503 | 250 | 9.4 | 5.0 | 300 |
| 259HY0505 | 250 | 9.4 | 5.0 | 500 |
| 259HY0510 | 250 | 9.4 | 5.0 | 1000 |
| 259HY1201 | 250 | 9.4 | 12.0 | 100 |
| 259HY1203 | 250 | 9.4 | 12.0 | 300 |
| 259HY1215 | 250 | 9.4 | 12.0 | 1500 |
| 3.52HY05006 | 35 | 2.1 | 5.0 | 60 |
| 3.52HY0501 | 35 | 2.1 | 5.0 | 100 |
| 3.52HY0502 | 35 | 2.1 | 5.0 | 200 |
| 3.52HY0503 | 35 | 2.1 | 5.0 | 300 |
| 3.52HY0505 | 35 | 2.1 | 5.0 | 500 |
| 3.52HY0510 | 35 | 2.1 | 5.0 | 1000 |
| 3.54HY03006 | 35 | 4.6 | 3.0 | 60 |
| 3.54HY0301 | 35 | 4.6 | 3.0 | 100 |
| 3.54HY0305 | 35 | 4.6 | 3.0 | 500 |
| 3.54HY0315 | 35 | 4.6 | 3.0 | 1500 |
| 3.54HY05006 | 35 | 4.6 | 5.0 | 60 |
| 3.54HY0501 | 35 | 4.6 | 5.0 | 100 |
| 3.54HY0502 | 35 | 4.6 | 5.0 | 200 |
| 3.54HY0503 | 35 | 4.6 | 5.0 | 300 |
| 3.54HY0505 | 35 | 4.6 | 5.0 | 500 |
| 3.54HY0510 | 35 | 4.6 | 5.0 | 1000 |
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