PolySULFOETHYL A
– 101 columnsThis strong cation-exchange (SCX) material was developed specifically for HPLC of peptides. At pH 2.7-3.0, peptides lose their (-) charges and have net (+) charge. Thus, PolySULFOETHYL A is a general-purpose alternative to reversed-phase (RPC), fractionating peptides by differences in charge rather than polarity. Compared to other SCX materials based on sulfopropyl- (SP-) groups, PolySULFOETHYL A is unusually hydrophilic. This minimizes hydrophobic interactions with peptides, with high recovery and less peak tailing. Capacity is also high, permitting better retention and fractionation of the weakly basic peptides from tryptic digests. The capacity of an ion-exchange material like this is 4x that of a comparable RPC column. Therefore, ion-exchange should be the initial step of a multi-step purification.
Use PolySULFOETHYL A for:
- Multidimensional HPLC of peptide mixtures, such as tryptic digests in proteomics analyses (including iTRAQ® and ICAT®* reaction products).
- Isolation of peptides from natural products.
- QC and purification of synthetic peptides.
- Selective isolation of disulfide-linked peptides, phosphopeptides and C-terminal fragments from tryptic digests.
- Mapping of peptide digests (tryptic, V8, CNBr, etc.).
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Brand specifications
Every parameter recorded for every column in this Brand is shown here. A single value applies across the Brand; multiple values show what is recorded across its columns.
| Mode | Ion Exchange | Pore Size (Å) | 100 / 1000 / 200 / 300 |
|---|---|---|---|
| Modification | Silica/SCX | Operating Temperature | Ambient |
Choose column specifications
Columns in this brand
Use the selectors above to narrow the list by specification, or search here by P/N or any displayed value.
| 050.05SE0502 | 50 | 0.05 | 5.0 | 200 |
| 050.05SE0503 | 50 | 0.05 | 5.0 | 300 |
| 050.05SE0510 | 50 | 0.05 | 5.0 | 1000 |
| 050.10SE0502 | 50 | 0.1 | 5.0 | 200 |
| 050.10SE0503 | 50 | 0.1 | 5.0 | 300 |
| 050.10SE0510 | 50 | 0.1 | 5.0 | 1000 |
| 050.17SE0502 | 50 | 0.175 | 5.0 | 200 |
| 050.17SE0503 | 50 | 0.175 | 5.0 | 300 |
| 050.17SE0510 | 50 | 0.175 | 5.0 | 1000 |
| 050.22SE0502 | 50 | 0.22 | 5.0 | 200 |
| 050.22SE0503 | 50 | 0.22 | 5.0 | 300 |
| 050.22SE0510 | 50 | 0.22 | 5.0 | 1000 |
| 050.32SE0502 | 50 | 0.32 | 5.0 | 200 |
| 050.32SE0503 | 50 | 0.32 | 5.0 | 300 |
| 050.32SE0510 | 50 | 0.32 | 5.0 | 1000 |
| 051SE0303 | 50 | 1.0 | 3.0 | 300 |
| 051SE0502 | 50 | 1.0 | 5.0 | 200 |
| 051SE0503 | 50 | 1.0 | 5.0 | 300 |
| 051SE0510 | 50 | 1.0 | 5.0 | 1000 |
| 054.0SE0501 | 50 | 4.0 | 5.0 | 100 |
| 054.0SE0502 | 50 | 4.0 | 5.0 | 200 |
| 054.0SE0503 | 50 | 4.0 | 5.0 | 300 |
| 054.0SE0510 | 50 | 4.0 | 5.0 | 1000 |
| 054SE0502 | 50 | 4.6 | 5.0 | 200 |
| 054SE0503 | 50 | 4.6 | 5.0 | 300 |
| 054SE0510 | 50 | 4.6 | 5.0 | 1000 |
| 100.05SE0502 | 100 | 0.05 | 5.0 | 200 |
| 100.05SE0503 | 100 | 0.05 | 5.0 | 300 |
| 100.05SE0510 | 100 | 0.05 | 5.0 | 1000 |
| 100.10SE0502 | 100 | 0.1 | 5.0 | 200 |
| 100.10SE0503 | 100 | 0.1 | 5.0 | 300 |
| 100.10SE0510 | 100 | 0.1 | 5.0 | 1000 |
| 100.17SE0502 | 100 | 0.175 | 5.0 | 200 |
| 100.17SE0503 | 100 | 0.175 | 5.0 | 300 |
| 100.17SE0510 | 100 | 0.175 | 5.0 | 1000 |
| 100.22SE0502 | 100 | 0.22 | 5.0 | 200 |
| 100.22SE0503 | 100 | 0.22 | 5.0 | 300 |
| 100.22SE0510 | 100 | 0.22 | 5.0 | 1000 |
| 100.32SE0502 | 100 | 0.32 | 5.0 | 200 |
| 100.32SE0503 | 100 | 0.32 | 5.0 | 300 |
| 100.32SE0510 | 100 | 0.32 | 5.0 | 1000 |
| 102SE0303 | 100 | 2.1 | 3.0 | 300 |
| 102SE0502 | 100 | 2.1 | 5.0 | 200 |
| 102SE0503 | 100 | 2.1 | 5.0 | 300 |
| 102SE0510 | 100 | 2.1 | 5.0 | 1000 |
| 104.0SE0502 | 100 | 4.0 | 5.0 | 200 |
| 104.0SE0503 | 100 | 4.0 | 5.0 | 300 |
| 104.0SE0510 | 100 | 4.0 | 5.0 | 1000 |
| 104SE0303 | 100 | 4.6 | 3.0 | 300 |
| 104SE0502 | 100 | 4.6 | 5.0 | 200 |
| 104SE0503 | 100 | 4.6 | 5.0 | 300 |
| 104SE0510 | 100 | 4.6 | 5.0 | 1000 |
| 109SE0303 | 100 | 9.4 | 3.0 | 300 |
| 109SE0502 | 100 | 9.4 | 5.0 | 200 |
| 109SE0503 | 100 | 9.4 | 5.0 | 300 |
| 109SE0510 | 100 | 9.4 | 5.0 | 1000 |
| 150.05SE0502 | 150 | 0.05 | 5.0 | 200 |
| 150.05SE0503 | 150 | 0.05 | 5.0 | 300 |
| 150.05SE0510 | 150 | 0.05 | 5.0 | 1000 |
| 150.10SE0502 | 150 | 0.1 | 5.0 | 200 |
| 150.10SE0503 | 150 | 0.1 | 5.0 | 300 |
| 150.10SE0510 | 150 | 0.1 | 5.0 | 1000 |
| 150.17SE0502 | 150 | 0.175 | 5.0 | 200 |
| 150.17SE0503 | 150 | 0.175 | 5.0 | 300 |
| 150.17SE0510 | 150 | 0.175 | 5.0 | 1000 |
| 150.22SE0502 | 150 | 0.22 | 5.0 | 200 |
| 150.22SE0503 | 150 | 0.22 | 5.0 | 300 |
| 150.22SE0510 | 150 | 0.22 | 5.0 | 1000 |
| 150.32SE0502 | 150 | 0.32 | 5.0 | 200 |
| 150.32SE0503 | 150 | 0.32 | 5.0 | 300 |
| 150.32SE0510 | 150 | 0.32 | 5.0 | 1000 |
| 151SE0502 | 150 | 1.0 | 5.0 | 200 |
| 151SE0503 | 150 | 1.0 | 5.0 | 300 |
| 151SE0510 | 150 | 1.0 | 5.0 | 1000 |
| 202SE0502 | 200 | 2.1 | 5.0 | 200 |
| 202SE0503 | 200 | 2.1 | 5.0 | 300 |
| 202SE0510 | 200 | 2.1 | 5.0 | 1000 |
| 204SE0502 | 200 | 4.6 | 5.0 | 200 |
| 204SE0503 | 200 | 4.6 | 5.0 | 300 |
| 204SE0510 | 200 | 4.6 | 5.0 | 1000 |
| 204SE1203 | 200 | 4.6 | 12.0 | 200 |
| 209SE0502 | 200 | 9.4 | 5.0 | 200 |
| 209SE0503 | 200 | 9.4 | 5.0 | 300 |
| 209SE0510 | 200 | 9.4 | 5.0 | 1000 |
| 209SE1203 | 200 | 9.4 | 12.0 | 200 |
| 2521SE0502 | 250 | 21.0 | 5.0 | 200 |
| 2521SE0503 | 250 | 21.0 | 5.0 | 300 |
| 2521SE0510 | 250 | 21.0 | 5.0 | 1000 |
| 2521SE1203 | 250 | 21.0 | 12.0 | 300 |
| 2550SE1203 | 250 | 50.8 | 12.0 | 300 |
| 259SE0502 | 250 | 9.4 | 5.0 | 200 |
| 259SE0503 | 250 | 9.4 | 5.0 | 300 |
| 259SE0510 | 250 | 9.4 | 5.0 | 1000 |
| 259SE1203 | 250 | 9.4 | 12.0 | 300 |
| 3.52SE0502 | 35 | 2.1 | 5.0 | 200 |
| 3.52SE0503 | 35 | 2.1 | 5.0 | 300 |
| 3.52SE0510 | 35 | 2.1 | 5.0 | 1000 |
| 3.54SE0303 | 35 | 4.6 | 3.0 | 300 |
| 3.54SE0502 | 35 | 4.6 | 5.0 | 200 |
| 3.54SE0503 | 35 | 4.6 | 5.0 | 300 |
| 3.54SE0510 | 35 | 4.6 | 5.0 | 1000 |