ZORBAX 300Extend
– 11 columns
ZORBAX 300Extend-C18: HPLC Columns for Biotechnology
ZORBAX 300Extend - C18 is a wide-bore HPLC column for high efficiency separations of peptides, polypeptides, and small proteins from pH 2-11.5. Extend-C18 and 300Extend-C18 columns incorporate a unique patented bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pH. At high pH, the retention and selectivity of peptides can change dramatically as a result of changes in amino acid charge. Excellent recoveries of hydrophobic polypeptides have been achieved at high pH with the 300Extend C18. In addition, LC/MS sensitivity of peptides can also be improved at high pH using a simple ammonium hydroxide mobile phase. These conditions allow for ions to be detected in both positive and negative ion modes simultaneously with the 300Extend-C18. The ZORBAX 300Extend-C18 is a rugged column and is ideal for LC/MS with ammonium hydroxide mobile phase.
- Rugged, high and low pH separations of peptides, polypeptides, and small proteins (pH 2 - 11.5)
- Different selectivity possible at high and low pH
- High efficiency and good recovery of hydrophobic peptides at high pH
- Ideal for LC/MS with ammonium hydroxide mobile phase
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Brand specifications
Every parameter recorded for every column in this Brand is shown here. A single value applies across the Brand; multiple values show what is recorded across its columns.
| Mode | Reversed-Phase | Endcapped | Yes |
|---|---|---|---|
| Modification | Silica/C18 | Operating Temperature | 60 |
| Pore Size (Å) | 300 | pH stability from | 2.0 |
| Surface Area | 45 | pH stability to | 11.5 |
| Carbon Load, % | 4.0 / 45.0 |
Choose column specifications
Columns in this brand
Use the selectors above to narrow the list by specification, or search here by P/N or any displayed value.
| 670750-902 | 75 | 2.0 | 5.0 | 300 |
| 671750-902 | 75 | 1.0 | 5.0 | 300 |
| 761775-902 | 100 | 2.0 | 3.5 | 300 |
| 761973-902 | 100 | 4.0 | 3.5 | 300 |
| 763750-902 | 150 | 2.0 | 3.5 | 300 |
| 763973-902 | 150 | 4.0 | 3.5 | 300 |
| 765750-902 | 50 | 2.0 | 3.5 | 300 |
| 765973-902 | 50 | 4.0 | 3.5 | 300 |
| 770995-902 | 250 | 4.0 | 5.0 | 300 |
| 773995-902 | 150 | 4.0 | 5.0 | 300 |
| 884950-571 | 150 | 4.0 | 3.5 | 300 |