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00D-4441-E0
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Clarity Oligo-RP

by Phenomenex

Purification and Analysis of Synthetic Oligonucleotides

The Clarity BioSolutions HPLC portfolio includes novel RP-HPLC and IEX-LC columns for purifiying and characterizing synthetic DNA / RNA used in biological research, therapuetic development, and biochemical manufacturing.

  • Clarity Oligo-MS™: LC/MS analysis
  • Clarity Oligo-RP™: RP-HPLC purification and analysis
  • Clarity Oligo-WAX™: IEX-LC purification


RP-HPLC Preparative Purification

Easily isolate target peak from N-1 / N+1 impurities to acheive >90 % purity of most synthetic oligonucleotides eliminating the need for extra purification steps.

  • 3 µm, 5 µm, 10 µm particles for seamless scaling
  • Cut larger fractions due to baseline resolution
  • Increased loadability compared to ion exchange

                                         OR


Highly Selective RP-HPLC Analysis

Baseline separate DNA or RNA strands with slightly different sequences to resolve difficult synthetic mixtures for easier quantitation.

  • Analyze oligos up to 60mer
  • High pH (1-12) and temperature stability (80 °C)
  • Hydrophobic, dipoloar, pi-pi, hydrogen bonding of sorbent facilitates unique selectivity
Specification
Vendor Phenomenex Length
Brand Clarity Oligo-RP Internal Diameter
Mode Reversed-Phase Pore size 110
Particle Size
Similar columns from other manufacturers to Clarity Oligo-RP

Up to 10 unique Brand families from other Vendors with the same Mode and the same Modification.

How similarity is calculated
Eligible families receive a weighted score from Modification (25%), Product Usage (20%), Carbon Load (15%), Endcapping (10%), Pore Size (10%), Surface Area (8%), pH Stability (8%) and Particle Size (4%). The score uses data available in both families and requires at least three comparable criteria, at least 50% data coverage and a minimum 55% score. A relevant active sponsor may appear first and is always labelled Sponsored.
A similarity score does not guarantee identical selectivity or performance. Confirm suitability under the intended method conditions.
No other-Vendor Brand family with the same Mode and Modification meets the comparable-data and similarity thresholds.
Product Usage values
Oligonucleotides
Application values
No Application values are assigned to this Product.
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