Product no. 58201
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Catalog SUPELCOSIL LC-Diol 58201

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SUPELCOSIL LC-Diol

by Sigma Aldrich

SUPELCOSIL™ LC-Diol HPLC Column
5 μm particle size, L × I.D. 25 cm × 3.0 mm
 
SUPELCOSIL™ HPLC Columns
Traditional HPLC columns, similar to Hypersil™
Our SUPELCOSIL silica-based HPLC column line includes over 40 bonded phase chemistries, in a range of particle sizes and column configurations from microbore to preparative scale. It is the original high quality Supelco product line referenced in many USP methods.
In the case of a few SUPLECOSIL phases, we have recommended alternatives based on more advanced column technology.

LC-Diol
Suitable for L20 per USP
LC-Diol columns can be used to separate proteins by gel filtration chromatography; when operated with an aqueous buffer, the diol phase shields the silica surface from interacting with proteins. The separation of steroids and sterols is commonly done using a diol column under normal phase conditions.

Specification

View this column family
Vendor Sigma Aldrich Pore size 120
Brand SUPELCOSIL LC-Diol Surface Area 170
Modifification Silica/Diol Carbon Load, % 3.5
Mode Reversed-Phase Endcapped No
Particle Size 5.0 Operating Temperature 70
Length 250 pH stability from 2.0
Internal Diameter 4.6 pH stability to 7.5

Product Usage

Proteins Steroids

Applications of column use

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