Vendor	Sigma Aldrich
Modifification	Silica/Amino
Mode	Reversed-Phase
Particle Size	5.0
Length	250
Internal Diameter	4.6
Brand	apHera NH2

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Catalog apHera NH2 56403AST

56403AST

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apHera NH2

by Sigma Aldrich

apHera™ NH2 HPLC Column
5 μm particle size, L × I.D. 15 cm × 2.0 mm

apHera™ Amino (NH2) Columns
apHera™ amino columns are based on covalently bonded polyamine specifically optimized for the separation of mono- and oligosaccharides. The elution order mono-, di-, tri-saccharide shows increased eluton volume with increased acetonitrile concentration and complete stability for both acidic and alkaline eluates. The small, robust PVA copolymer bead provides mechanical and chemical strength as well as high column efficiency. Conventional camion columns based on silica do not show long column life, perhaps due to hydrolysis of silica particle by the basic amino group. Since Supelco uses a strong alkaline compatible polymer, these problems are eliminated. Stable retention time and long column life are also characteristic of the column.

Features of Amino apHera™ Columns

High efficiency for carbohydrate analysis
Ideal for basic conditions/amphoteric detection
High selectivity mono- to oligosaccharides
pH range 2-13
300 angstrom pore size
Stable, predictable retention
Long column life

Specification

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Vendor	Sigma Aldrich	Particle Size	5.0
Brand	apHera NH2	Length	250
Modifification	Silica/Amino	Internal Diameter	4.6
Mode	Reversed-Phase

Product Usage

Monosaccharides/disaccharides Oligosaccharides

Applications of column use

Amino Acids, Biochemistry

Similar columns from other manufacturers to apHera NH2

Up to 10 unique Brand families from other Vendors with the same Mode and the same Modification.

How similarity is calculated

Eligible families receive a weighted score from Modification (25%), Product Usage (20%), Carbon Load (15%), Endcapping (10%), Pore Size (10%), Surface Area (8%), pH Stability (8%) and Particle Size (4%). The score uses data available in both families and requires at least three comparable criteria, at least 50% data coverage and a minimum 55% score. A relevant active sponsor may appear first and is always labelled Sponsored.

A similarity score does not guarantee identical selectivity or performance. Confirm suitability under the intended method conditions.

No other-Vendor Brand family with the same Mode and Modification meets the comparable-data and similarity thresholds.

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