Ascentis® Express Phenyl-Hexyl HPLC Column
2.7 μm particle size, L × I.D. 10 cm × 2.1 mm        

Ascentis® Express Phenyl-Hexyl HPLC Columns
The phenyl phase has unique reversed-phase selectivity, especially for polar aromatics and heterocyclic compounds, arising from solute interaction with the aromatic ring and its delocalized electrons. Ascentis™ Express Phenyl-Hexyl is complementary (orthogonal) to both C18 and RP-Amide Ascentis Express phases because of this unique aromaticity. Phenyl phases also tend to exhibit good shape selectivity, which may originate from solute multipoint interaction with the planar ring system. More retention and selectivity will often be observed for solutes with aromatic electron-withdrawing groups (fluorine, nitro, etc.) or with a delocalized heterocyclic ring system such as the benzodiazepine compounds.