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apHera C18

by Sigma Aldrich

apHera™ C18 HPLC Column
5 μm particle size, L × I.D. 15 cm × 2.0 mm

apHera™ C18 Reversed-phase Columns
apHera™ reversed columns were devoloped specifically to provide the superior advantages of both silica and polystyrene columns, without the disadvantages of either. This was accomplished using a vinyl alcohol copolymer base that keeps the surface wetted even with high carbon loads. The porous structure has an average pore diameter large enough to produce ideal results for small analytes, peptides and small proteins. These columns equal silica based columns in separation efficiency with organic solvents but provide efficiency with buffered alkaline solutions not possible on silica. One of the most significant features is logicial elution order of alkylated bases where retention increases proportionately with increasing chain length.

Features of apHera™ Reversed Phase Columns

Stable vinyl copolymer base
Stable pH range 2-12
300 angstrom pore size
Stable in all organic solvents
Expanded applications: peptides, proteins
Efficient separation of any basic substance
Amenability to washing and alkaline solutions

Specification

Vendor	Sigma Aldrich	Particle Size
Brand	apHera C18	Length
Mode	Reversed-Phase	Internal Diameter

Similar columns from other manufacturers to apHera C18

Up to 10 unique Brand families from other Vendors with the same Mode and the same Modification.

How similarity is calculated

Eligible families receive a weighted score from Modification (25%), Product Usage (20%), Carbon Load (15%), Endcapping (10%), Pore Size (10%), Surface Area (8%), pH Stability (8%) and Particle Size (4%). The score uses data available in both families and requires at least three comparable criteria, at least 50% data coverage and a minimum 55% score. A relevant active sponsor may appear first and is always labelled Sponsored.

A similarity score does not guarantee identical selectivity or performance. Confirm suitability under the intended method conditions.

No other-Vendor Brand family with the same Mode and Modification meets the comparable-data and similarity thresholds.

Product Usage values

Peptides Proteins Small molecules

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