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568422-U
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Discovery BIO Wide Pore C5

by Sigma Aldrich

Discovery® BIO Wide Pore C5 HPLC Column
3 μm particle size, L × I.D. 10 cm × 0.32 mm

Discovery BIO Wide Pore C5
HPLC Columns and Capillaries
Discovery BIO Wide Pore C5 was designed for the efficient and reliable separation of proteins and peptides, especially hydrophobic peptides, by RP- HPLC. Long-chain phases, like C8 or C18, are often too hydrophobic for proteins and can cause excessively long retention time or even irreversible binding to the column. For this reason short-chain phases, typically C3 or C4, are often used for RP-HPLC of proteins. However, these short-chain phases are susceptible to hydrolysis, resulting in short column lifetime, especially at low pH. The Discovery BIO Wide Pore C5 gives elution order similar to a conventional C4, yet has enhanced pH stability for longer column lifetime. Generally, higher efficiency separations are achievable on the Discovery BIO Wide Pore C5 because of the improvements we have made to the silica and bonded-phase chemistry.

Specification
Vendor Sigma Aldrich Particle Size
Brand Discovery BIO Wide Pore C5 Length
Modifification Silica/C5 Internal Diameter
Mode Reversed-Phase
Similar columns from other manufacturers to Discovery BIO Wide Pore C5

Up to 10 unique Brand families from other Vendors with the same Mode and the same Modification.

How similarity is calculated
Eligible families receive a weighted score from Modification (25%), Product Usage (20%), Carbon Load (15%), Endcapping (10%), Pore Size (10%), Surface Area (8%), pH Stability (8%) and Particle Size (4%). The score uses data available in both families and requires at least three comparable criteria, at least 50% data coverage and a minimum 55% score. A relevant active sponsor may appear first and is always labelled Sponsored.
A similarity score does not guarantee identical selectivity or performance. Confirm suitability under the intended method conditions.
Jupiter C5
by Phenomenex
88% match
ModeReversed-Phase
ModificationSilica/C5
Pore Size300 Å
Shared Product UsageProteins, Hydrophobic compounds
View Brand
Product Usage values
Hydrophobic compounds Peptides Proteins
Application values
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