Fermentation Monitoring Column

Aminex HPLC columns are packed with a polymer-based matrix (polystyrene divinylbenzene). They separate compounds using the ion-moderated partition chromatography technique. Aminex carbohydrate analysis columns separate compounds using a combination of size exclusion and ligand exchange mechanisms:

In oligosaccharide separations, size exclusion is the primary mechanism. Low crosslinked resins allow carbohydrates to penetrate, and oligosaccharides separate by size.

This hydrogen-form, 150 x 7.8 mm column is optimized to resolve maltotriose, maltose, glucose, and fructose while also resolving acids and alcohols. When analysis of sugars in a fermentation broth is required, this is the column of choice. Dilute sulfuric acid is the only eluent used, and filtering is generally the only sample preparation required.