HALO PENTA-HILIC                                             

The Penta-HILIC is specifically designed and intended for hydrophilic interaction liquid chromatography, HILIC. The HALO Penta-HILIC stationary phase is a highly polar ligand that possesses 5 hydroxyl groups tethered to the silica via novel proprietary linkage chemistry. The retention mechanism offered by the Penta-HILIC phase is a combination of hydrophilic interaction, ion-exchange and weak hydrophobic binding interaction. Retention in HILIC mode as a function of mobile phase is opposite that of reversed phase. For example, increasing the mobile phase strength involves increasing the polarity of the mobile phase by increasing the aqueous component. Penta-HILIC is particularly recommended for the separation of highly polar analytes that are poorly retained in reversed phase mode. The Penta-HILIC phase is also recommended for LC-MS applications because of the MS friendly mobile phase conditions typically used with Penta-HILIC.