RP 5G is produced from highly cross-linked Polystyrene-divinyl benzene (PS-DVB) spherical beads. The surface of the materials has been modified by hydrophilic coating to reduce the hydrophobicity and the small pores of these particles have been sealed to prevent the trapping of biomolecules. These supports are suitable for reversed phase analysis and purification of small polypeptides, oligonucleotides and proteins.

RP 5G is prepared from 5 µm, 500 Å spherical PS-DVB particles with hydrophilic surface coating. This polymer provides optimum surface area with high separating efficiencies for small drug, and polypeptides.

These materials are extremely stable and permit use of mobile phase pH from 1 to 14 and the maximum temperature limit is up to 80° C. Reversed phase chromatography is based on the discrimination by hydrophobicity of biomolecules and is a complementary technique to ion exchange and hydrophobic interaction chromatography.