Affinity Chromatography is a very powerful technique commonly used to purify a biomolecule of interest based on the specific interaction of the biomolecule with a ligand immobilized on a stationary phase. Affinity chromatography is widely used in biotechnology research and in industry. Using this technique, molecules of interest can be purified in a one-step separation with high purity and high recovery.

Orochem Glutathione Resin can be effectively used for purification of high levels of over expressed GST-tagged fusion proteins from bacterial lysates. Glutathione Resin is packed into HPLC column for multiple rounds of affinity purification.