Fermentation Monitoring Column
Aminex HPLC columns are packed with a polymer-based matrix (polystyrene divinylbenzene). They separate compounds using the ion-moderated partition chromatography technique. Aminex carbohydrate analysis columns separate compounds using a combination of size exclusion and ligand exchange mechanisms:
In oligosaccharide separations, size exclusion is the primary mechanism. Low crosslinked resins allow carbohydrates to penetrate, and oligosaccharides separate by size.
This hydrogen-form, 150 x 7.8 mm column is optimized to resolve maltotriose, maltose, glucose, and fructose while also resolving acids and alcohols. When analysis of sugars in a fermentation broth is required, this is the column of choice. Dilute sulfuric acid is the only eluent used, and filtering is generally the only sample preparation required.
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