Ascentis® Express HILIC HPLC Column
2.7 μm particle size, L × I.D. 10 cm × 2.1 mm    

Ascentis® Express HILIC HPLC Columns
Suitable for L3 per USP
HILIC chromatography is a variation of normal-phase chromatography with the distinction that one of the major components of the mobile phase is water. The high volatility of the mobile phase makes HILIC LC-MS friendly, and allows for increased MS sensitivity. Many classes of polar compounds can be retained on the Ascentis™ Express HILIC column; including, polar neutrals, polar acids, and polar and non-polar basic amines. The HILIC column provides orthogonal selectivity to the Ascentis Express C18, with the elution typically the opposite of that for C18. The non-polar compounds elute early and the highly polar compounds have greater retention and selectivity.

Ascentis Express HPLC columns, through the use of Fused-Core™ particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users.