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050.05HY05006

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050.05HY05006
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PolyHYDROETHYL A

by Poly LC Inc.

PolyHYDROXYETHYL A - for Hydrophilic Interaction and Size Exclusion HPLC

This neutral, polar material was developed specifically for Hydrophilic Interaction Chromatography (HILIC). HILIC is a variant of normal phase chromatography that is performed with a polar stationary phase and a partly aqueous mobile phase. This permits normal-phase separations of peptides, nucleic acids, carbohydrates, some proteins, and polar solutes in general. In comparison with other materials used for HILIC, PolyHYDROXYETHYL A affords sharper peaks and better selectivity and recovery. Its great polarity means less organic solvent is needed to get retention.

Use PolyHYDROXYETHYL A for:

  • Analysis of polar small molecules for metabolomics or analysis via HILIC-MS/MS of specific small molecules (amino acids; methotrexate) in plasma or crude extracts.
  • Peptide separations and mapping involving differences in polar groups (Ser-; glycopeptides; etc.).
  • Multidimensional purification of synthetic and natural peptides or fractionation of really complex digests.
  • HPLC of solutes that aren't soluble in aqueous media (membrane proteins;phospholipids).
  • Eliminating detergents, lipids, and salts from a sample.
  • Oligonucleotides and their analogs.
Specification
Vendor Poly LC Inc. Length
Brand PolyHYDROETHYL A Internal Diameter
Modifification Silica/ Polyhydroxyethyl Pore size 60
Mode HILIC/Size Exclusion Operating Temperature Ambient
Particle Size
Similar columns from other manufacturers to PolyHYDROETHYL A

Up to 10 unique Brand families from other Vendors with the same Mode and the same Modification.

How similarity is calculated
Eligible families receive a weighted score from Modification (25%), Product Usage (20%), Carbon Load (15%), Endcapping (10%), Pore Size (10%), Surface Area (8%), pH Stability (8%) and Particle Size (4%). The score uses data available in both families and requires at least three comparable criteria, at least 50% data coverage and a minimum 55% score. A relevant active sponsor may appear first and is always labelled Sponsored.
A similarity score does not guarantee identical selectivity or performance. Confirm suitability under the intended method conditions.
No other-Vendor Brand family with the same Mode and Modification meets the comparable-data and similarity thresholds.
Product Usage values
Amino acids Carbohydrate Lipids Nucleic acids Oligonucleotides Peptides Polar compounds Proteins Small molecules Synthetic peptides
Application values
No Application values are assigned to this Product.
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