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050.05SE0502

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050.05SE0502
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PolySULFOETHYL A

by Poly LC Inc.

This strong cation-exchange (SCX) material was developed specifically for HPLC of peptides. At pH 2.7-3.0, peptides lose their (-) charges and have net (+) charge. Thus, PolySULFOETHYL A is a general-purpose alternative to reversed-phase (RPC), fractionating peptides by differences in charge rather than polarity. Compared to other SCX materials based on sulfopropyl- (SP-) groups, PolySULFOETHYL A is unusually hydrophilic. This minimizes hydrophobic interactions with peptides, with high recovery and less peak tailing. Capacity is also high, permitting better retention and fractionation of the weakly basic peptides from tryptic digests. The capacity of an ion-exchange material like this is 4x that of a comparable RPC column. Therefore, ion-exchange should be the initial step of a multi-step purification.

Use PolySULFOETHYL A for:

  • Multidimensional HPLC of peptide mixtures, such as tryptic digests in proteomics analyses (including iTRAQ® and ICAT®* reaction products).
  • Isolation of peptides from natural products.
  • QC and purification of synthetic peptides.
  • Selective isolation of disulfide-linked peptides, phosphopeptides and C-terminal fragments from tryptic digests.
  • Mapping of peptide digests (tryptic, V8, CNBr, etc.).
Specification
Vendor Poly LC Inc. Length
Brand PolySULFOETHYL A Internal Diameter
Modifification Silica/SCX Pore size 200
Mode Ion Exchange Operating Temperature Ambient
Particle Size
Similar columns from other manufacturers to PolySULFOETHYL A

Up to 10 unique Brand families from other Vendors with the same Mode and the same Modification.

How similarity is calculated
Eligible families receive a weighted score from Modification (25%), Product Usage (20%), Carbon Load (15%), Endcapping (10%), Pore Size (10%), Surface Area (8%), pH Stability (8%) and Particle Size (4%). The score uses data available in both families and requires at least three comparable criteria, at least 50% data coverage and a minimum 55% score. A relevant active sponsor may appear first and is always labelled Sponsored.
A similarity score does not guarantee identical selectivity or performance. Confirm suitability under the intended method conditions.
HECTOR-ACD SCX
by RSTech
58% match
ModeIon Exchange
ModificationSilica/SCX
Pore Size100 Å
Shared Product UsageNo shared usage assigned
View Brand
Product Usage values
Peptides Synthetic peptides
Application values
No Application values are assigned to this Product.
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