150.32SE0510

Column specification sheet

Vendor	Poly LC Inc.
Modifification	Silica/SCX
Mode	Ion Exchange
Particle Size	5.0
Length	150
Internal Diameter	0.32
Pore size	1000
Operating Temperature	Ambient
Brand	PolySULFOETHYL A

Product Usage

Peptides, Synthetic peptides

https://hplcmart.com/product/58017

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Catalog PolySULFOETHYL A 150.32SE0510

150.32SE0510

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PolySULFOETHYL A

by Poly LC Inc.

This strong cation-exchange (SCX) material was developed specifically for HPLC of peptides. At pH 2.7-3.0, peptides lose their (-) charges and have net (+) charge. Thus, PolySULFOETHYL A is a general-purpose alternative to reversed-phase (RPC), fractionating peptides by differences in charge rather than polarity. Compared to other SCX materials based on sulfopropyl- (SP-) groups, PolySULFOETHYL A is unusually hydrophilic. This minimizes hydrophobic interactions with peptides, with high recovery and less peak tailing. Capacity is also high, permitting better retention and fractionation of the weakly basic peptides from tryptic digests. The capacity of an ion-exchange material like this is 4x that of a comparable RPC column. Therefore, ion-exchange should be the initial step of a multi-step purification.

Use PolySULFOETHYL A for:

Multidimensional HPLC of peptide mixtures, such as tryptic digests in proteomics analyses (including iTRAQ® and ICAT®* reaction products).
Isolation of peptides from natural products.
QC and purification of synthetic peptides.
Selective isolation of disulfide-linked peptides, phosphopeptides and C-terminal fragments from tryptic digests.
Mapping of peptide digests (tryptic, V8, CNBr, etc.).

Specification

View this column family

Vendor	Poly LC Inc.	Length	150
Brand	PolySULFOETHYL A	Internal Diameter	0.32
Modifification	Silica/SCX	Pore size	1000
Mode	Ion Exchange	Operating Temperature	Ambient
Particle Size	5.0

Product Usage

Peptides Synthetic peptides

Applications of column use

No Applications are assigned to this Product.

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How similarity is calculated

Eligible families receive a weighted score from Modification (25%), Product Usage (20%), Carbon Load (15%), Endcapping (10%), Pore Size (10%), Surface Area (8%), pH Stability (8%) and Particle Size (4%). The score uses data available in both families and requires at least three comparable criteria, at least 50% data coverage and a minimum 55% score. A relevant active sponsor may appear first and is always labelled Sponsored.

A similarity score does not guarantee identical selectivity or performance. Confirm suitability under the intended method conditions.

HECTOR-ACD SCX

by RSTech

58% match

ModeIon Exchange

ModificationSilica/SCX

Pore Size100 Å

Shared Product UsageNo shared usage assigned

View Brand

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