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720402.4

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720402.4
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428.00
per column · ex. VAT

NUCLEOSIL PEI

by MACHEREY-NAGEL

NUCLEOSIL® standard silica phases for HPLC

Features

NUCLEOSIL® is a family of totally porous spherical silicas with a very pure and uniform SiO2 structure

  • Wide acceptance as routine packings for very different fields of chromatography
  • One of the first spherical silicas used in HPLC
  • Developed in the early seventies, it became a world-renowned HPLC packing
  • Still found in many analytical and preparative applications
  • An absolutely reliable choice in HPLC
  • The largest variety of modified HPLC silicas available on the market
     

NUCLEOSIL® 4000-7 PEI  
Silica-based HPLC phase for anion exchange chromatography of proteins and peptides
 
Features
 

  •  Base material NUCLEOSIL® silica, particle size 7 µm, pore size 4000 Å polymeric, covalently bonded polyethyleneimine network, weakly basic anion exchanger
  • Ion exchange capacity 0.15 mmol/g; protein binding capacity 61 mg BSA/g; pH stability 2–8.5; max. working pressure 250 bar
  • Separation principle: reversible adsorption of negatively charged substances to positively charged groups on the exchanger material and their subsequent displacement by either increasing ionic strength or pH changes in the mobile phase
  • Eluent in column methanol   
  •  High selectivity for numerous proteins: e.g., β-lactoglobulins A and B, two proteins differing in just two amino acids, can be separated in only 10 minutes; biological activity of purified proteins is preserved
  • Good binding and desorption kinetics for nucleotides as well
Specification
Vendor MACHEREY-NAGEL Pore size 4000
Brand NUCLEOSIL PEI Max Pressure 3625
Modifification Silica/WAX Surface Area 10
Mode Anion-Exchange Endcapped N/A
Particle Size 7.0 Operating Temperature 60
Length 125 pH stability from 2.0
Internal Diameter 4.0 pH stability to 8.5
Similar columns from other manufacturers to NUCLEOSIL PEI

Up to 10 unique Brand families from other Vendors with the same Mode and the same Modification.

How similarity is calculated
Eligible families receive a weighted score from Modification (25%), Product Usage (20%), Carbon Load (15%), Endcapping (10%), Pore Size (10%), Surface Area (8%), pH Stability (8%) and Particle Size (4%). The score uses data available in both families and requires at least three comparable criteria, at least 50% data coverage and a minimum 55% score. A relevant active sponsor may appear first and is always labelled Sponsored.
A similarity score does not guarantee identical selectivity or performance. Confirm suitability under the intended method conditions.
No other-Vendor Brand family with the same Mode and Modification meets the comparable-data and similarity thresholds.
Product Usage values
No Product Usage values are assigned to this Product or its Brand.
Application values
No Application values are assigned to this Product.
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